Hi guys, i'm an undergraduate biochem student and I can't get me head round some of the illumina system.
Can anybody please put it to me in laymans language, things like:
1. Are the adapters attached on the DNA fragments used as primers??
2. Are the adapters unique for each and every library fragment??
3. What influences the formation of the bridge during the polymerization??
4. How do the reversible terminators work in this respect??
5. What is the capacity of one flow cell??
Can anybody please put it to me in laymans language, things like:
1. Are the adapters attached on the DNA fragments used as primers??
2. Are the adapters unique for each and every library fragment??
3. What influences the formation of the bridge during the polymerization??
4. How do the reversible terminators work in this respect??
5. What is the capacity of one flow cell??
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