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Old 01-22-2013, 07:08 AM   #1
DNADEB
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Location: PA

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Default Single Cell Ampliseq?

We are developing a workflow for using the Ampliseq Cancer Panel starting with DNA from single cells. We plan to use WGA but wonder if someone could recommend a kit. Genomiphi for example requires 10 ngs. A single cell will have about 6 pgs. Any advice?
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Old 01-22-2013, 01:26 PM   #2
cliffbeall
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The following paper has a method to purify the Phi29 polymerase away from DNA contamination, which can be a problem when amplifying low concentrations of DNA. I am not sure if there is a commercial product yet.

Blainey, P. C. and S. R. Quake (2011). "Digital MDA for enumeration of total nucleic acid contamination." Nucleic Acids Research 39(4) e19.
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Old 01-23-2013, 06:36 AM   #3
kwaraska
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Qiagen has just introduced a product that is a single cell WGA.

Don't have any experience with it but might be a good starting point.
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