I usually use 1x TE but made a mistake and used Illumina's RSB instead. First off, will the shearing still work? And second, if I try to ampure cleanup the sample after shearing, will the DNA bind to the beads? Since it's an elution buffer I'm worried that it wouldn't bind to the beads and I would lose all my material. I typically shear in 130ul and add 180ul of ampure and then elute in RSB.
Any advice or thoughts, good or bad, would be helpful. I don't know what to think, but I'm leaning towards it being bad. Not feeling to great.
Any advice or thoughts, good or bad, would be helpful. I don't know what to think, but I'm leaning towards it being bad. Not feeling to great.
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