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Old 11-26-2008, 10:01 AM   #1
Location: Ames, IA

Join Date: Nov 2008
Posts: 57
Cool Centromere (just wondering)

I understand that we can either fractionate RNA or cDNA so poly A library is not a necessity for RNA-seq. However, would fractionated RNA alone serve to give a decent coverage?

Last edited by Siva; 11-26-2008 at 02:20 PM. Reason: Learnt more about RNA-seq
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Old 07-04-2011, 03:22 PM   #2
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Location: Sydney Australia

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PolyA selection allows for selection of mRNA. Total RNA from a cell/tissue contains more than 98% of ribosomal RNA sequences and therefore it would be wise to do either polyA selection or remove ribosomal RNA by other means. If not done, you end up having 80% of your reads mappings to ribosomes.
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