Hi all,
I am using (for the first time) featureCounts to get gene level counts in a RNAseq experiment... It is mouse RNAseq and polyA RNA were extracted...
A quick look at the results shows up to 15% of reads which are unassigned because of no overlap with any feature.
Do you normally have the same stats?
s.
I am using (for the first time) featureCounts to get gene level counts in a RNAseq experiment... It is mouse RNAseq and polyA RNA were extracted...
A quick look at the results shows up to 15% of reads which are unassigned because of no overlap with any feature.
Do you normally have the same stats?
s.
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