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06-17-2011, 01:58 AM
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#1 |
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Junior Member
Location: cambridge Join Date: Jun 2011
Posts: 1
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primer/adapter sequences
Hi,
can anyone please help??? I have been using the Illumina ChIP-Seq amplification kit for a while now and would like to move to the NEB kit. The NEB kit doesn't contain the primers/adapters and they suggested looking on this forum for the sequences, can anyone help???? Where would you normally order them from?? Do they need to be HPLC purified???? Many thanks |
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06-21-2011, 12:42 PM
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#2 |
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Junior Member
Location: Boston Join Date: Apr 2011
Posts: 1
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See the attached file. Use PE adapters and PE PCR primers on page 3. You can order them from IDT and choose PAGE purification.
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06-21-2011, 01:36 PM
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#3 |
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Senior Member
Location: USA Join Date: Apr 2009
Posts: 482
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Note that for best results the bond between the last 2 bases on the 3' end must be a phosphothioate bond. Most oligo companies can do this. If you don't modify the oligo you will get adapter artifacts.
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