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Old 01-03-2012, 01:29 AM   #1
Senior Member
Location: Germany

Join Date: May 2010
Posts: 150
Unhappy directional vs. non-directional sequencing


Can someone please explain the difference between the directional and the non-directional sequencing methods?

I was looking for articles or sites which can explain it, especially the results one get from such run.

We have our results. This suppose to be a non-directional RNA-seq. The question was now, if we can extract the strand information from these data. I mean, is there a way to know if a strand belongs to the + or - strand?
In the bam file we have these results, but as far as I understand it, this information says only if the read was mapped to the forward or backward strand.

Am I wrong with this assumption?

I would appreciate ant information which will help me/us better understand the difference between the two technologies.

frymor is offline   Reply With Quote
Old 01-03-2012, 02:20 AM   #2
Location: Leeds, UK

Join Date: Apr 2010
Posts: 63

It's due to a difference in the library prep. Directional RNA-seq keeps the strand information by only making the cDNA in one direction, non-directional will make cDNA in both directions, so you have no way of knowing if your sequence read is in the same direction as the original transcription.
henry.wood is offline   Reply With Quote

directional rna-seq, directionality, non-directional rna-seq

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