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Conver mm8 WIG to mm9 WIG seqmagician Bioinformatics 0 05-07-2011 07:28 AM

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Old 03-21-2014, 01:31 AM   #1
Junior Member
Location: india

Join Date: Jun 2012
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Hello All,
NGS data from SRA is downloaded in form of .sra files which are highly compressed and save a lot of space. fastq-dump converts .sra to fastq. i am interested in something which converts fastq, back to sra, i learnt fastq-load does it, but cannot find any manual explaining how to. Does anyone know how to and can help?
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Old 03-21-2014, 02:58 AM   #2
David Eccles (gringer)
Location: Wellington, New Zealand

Join Date: May 2011
Posts: 835

well, running the help from the command line will probably give you quite a lot of help:
$ fastq-load --help

        fastq-load [options] -r run.xml -e experiment.xml -o output-path

  -r|--run-xml                     path to run.xml describing input files 
  -e|--experiment                  path to experiment.xml 
  -o|--output-path                 target location 

  -i|--input-path                  input files location, default '.' 
  -u|--input-unpacked              input files are unpacked 
... etc.
The XML schemas for Run / Experiment can be found on the NCBI SRA website.
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