Our machine was prepped for a Paired End run, but we forgot to enter the read length for the paired read, so the machine ran it through as single end.
The job has completed, and the flow cell is sitting there.... it seems that all we would need to do is convince the Illumina software to turn the molecules over and start the second run.
Illumina tech support say this is impossible.
Does anyone know how to save this run?
I renamed/move all of the files produced during the run (netcopy files, Processing & Data directories), modified the runParameters.xml and restarted the program, hoping to resume a sequencing run, but it didn't work.
Edit: Idea
We have thought of an idea, which is to do a second run, with 0 bases (or 1) for the first read, then continue on with the pair. We can then match reads from the 1st run with the 2nd via tile ID.
Does anyone have any comments on whether this would work?
The job has completed, and the flow cell is sitting there.... it seems that all we would need to do is convince the Illumina software to turn the molecules over and start the second run.
Illumina tech support say this is impossible.
Does anyone know how to save this run?
I renamed/move all of the files produced during the run (netcopy files, Processing & Data directories), modified the runParameters.xml and restarted the program, hoping to resume a sequencing run, but it didn't work.
Edit: Idea
We have thought of an idea, which is to do a second run, with 0 bases (or 1) for the first read, then continue on with the pair. We can then match reads from the 1st run with the 2nd via tile ID.
Does anyone have any comments on whether this would work?
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