Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Does bwa support SOLiD PE data?

    Hi,
    I have used 'bwa aln' and 'bwa sampe' for mapping Exome captured SOLiD PE reads.
    I am getting very low PE mapping % (29-35%) One of the notes on the 'bwa version 0.5.9-r16' says:
    Notes:
    1. For SOLiD reads, <in1.fq> corresponds R3 reads and <in2.fq> to F3.
    2. For reads shorter than 30bp, applying a smaller -o is recommended to
    to get a sensible speed at the cost of pairing accuracy.

    So my question is if bwa truly support SOLiD PE reads?
    If so, what parameters have to be modified ?
    The reads are F3 and F5, there is no R3.

    Do the version 0.5.9-r16 have changes to sampe that was posted by Brugger at bwa-support mailing list.

    CATEGORY TOTAL_READS PF_READS %_PF_READS PF_NOISE_READS PF_READS_ALIGNED %_PF_READS_ALIGNED
    FIRST_OF_PAIR 134520572 134520572 1 34 14456389 0.107466
    SECOND_OF_PAIR 134520572 134520572 1 17 82292274 0.611745
    PAIR 269041144 269041144 1 51 96748663 0.359605

    CATEGORY READS_ALIGNED_IN_PAIRS %_READS_ALIGNED_IN_PAIRS
    FIRST_OF_PAIR 10169637 0.70347
    SECOND_OF_PAIR 10169650 0.12358
    PAIR 20339287 0.210228



    Thanks
    VJ
    Last edited by 2007lab; 05-09-2011, 12:20 PM. Reason: bwa spelling

Latest Articles

Collapse

  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin


    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...
    Yesterday, 07:01 AM
  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
54 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
50 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 09:21 AM
0 responses
44 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-04-2024, 09:00 AM
0 responses
55 views
0 likes
Last Post seqadmin  
Working...
X