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I'm having a lot of issues with NuGEN's Ovation 3'DGE and RNA-Seq library preparation kits. No matter what I do, I can't seem to generate any libraries for sequencing. Has anyone had a similar problem? When I use the mRNA Seq prep kit from Illumina and try it on the same samples I'm getting great libraries....hmmm.....frustrated....
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are you using Illumina kit to generate library itself, or your own reagents? Anyway, there is a report in Biotechniques that shows that pretreatment of ovation-generated ds cDNA with S1 nuclease increases efficiency of library generation. Their claim is that it's due to hairpins at the ends of DNA fragments that obviously would affect any end repair /adaptor ligation. S1 ligase will get rid of the ssDNA, so should solve the problem.
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...Today, 07:48 AM -
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