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Old 05-17-2017, 03:36 AM   #21
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Have anyone try analysis the error rate of per base in amplicons? We found lots of sites with error rate larger than 0.01, any idea why this happen?
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Old 10-03-2017, 02:43 PM   #22
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Has anyone gotten this protocol to work? I have a protocol that uses Phusion U MM (thanks to epistatic) that appears to work, but I haven't tried it without the end repair reaction yet. Do you think the end repair is necessary?

Last edited by Buckethead84; 11-24-2017 at 10:35 PM.
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ampliseq, miseq

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