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Old 09-21-2017, 09:20 PM   #1
Sacrolfur
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Post cutPrimers: new tool for removing primer sequences from targeted NGS reads

I present you new tool for removing primer sequences from NGS reads that were obtained during sequencing amplicon-based targeted panel. You can download it from https://github.com/aakechin/cutPrimers. It runs under any OS: Linux, Mac OS, Windows. If you have any question, you can ask me.

Now, cutPrimers remove primer sequences only from unmapped reads in FASTQ-files. Later, it will be able to cut primer sequences also from mapped reads.

I will be glad to answer for any questions about its use.
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Old 09-21-2017, 09:31 PM   #2
ankitgoyal
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Thanks for updating. Does this work on every windows version?
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Old 09-21-2017, 09:40 PM   #3
Sacrolfur
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I tried it only on the Windows 7. Thank you for the question. I'll try it on the Windows XP and Windows 10
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Old 09-21-2017, 10:01 PM   #4
ankitgoyal
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Thanks for replying. Please let me know once you get some results of trying it on other OS. I had to discuss this with my Boss.
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Old 09-26-2017, 12:10 AM   #5
Sacrolfur
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I fixed some bugs for using in Windows. And I approved that it works now in Windows 7 and Windows 10. Windows XP will be a little bit later
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Old 09-27-2017, 07:49 AM   #6
Sacrolfur
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Unfortunately, cutPrimers does not work in Windows XP, because in Windows XP you can not install Python 3.6 and can not install Biopython module, because you can not install Visual Studio C++
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