Has anyone tried to save and sequence the polyA(-) supernatant from Illumina's TruSeq stranded mRNA library prep kit? If so, any advice or insight into things to consider using the supernatant - ie: would I do another phenol extraction or something to pellet out the polyA(-) RNA then suspend in H2O?
I'm interested in small RNAs (miRNA, piRNA) in particular so I assume I'd need to clean up the supernatant on a gel to size select for 20-30 nt or maybe use another rRNA depletion to clean up the samples.
Thanks!
This kit:
I'm interested in small RNAs (miRNA, piRNA) in particular so I assume I'd need to clean up the supernatant on a gel to size select for 20-30 nt or maybe use another rRNA depletion to clean up the samples.
Thanks!
This kit:
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