Hi everybody,
Recently i came across with the contamination with he PCR kits while i was trying to amplify the 16s Amplicons using V1-V2 primers. i have used Takara and Bioneer kits. But both of them gives a very prominent band even with the negative controls(primers only). i did the capillary sequencing for the negative bands, which showed the presence of some uncultured strains and e.coli.. etc....
I even tried treating them with Sau3A1. But then after de-activating Sau3A1, the taq polymerase becomes inactive....
Can anyone suggest me a good kit for the 16s Amplicon sequencing...
Please share ur experiences if anyone came across with such problems
Thank you
Rosh
Recently i came across with the contamination with he PCR kits while i was trying to amplify the 16s Amplicons using V1-V2 primers. i have used Takara and Bioneer kits. But both of them gives a very prominent band even with the negative controls(primers only). i did the capillary sequencing for the negative bands, which showed the presence of some uncultured strains and e.coli.. etc....
I even tried treating them with Sau3A1. But then after de-activating Sau3A1, the taq polymerase becomes inactive....
Can anyone suggest me a good kit for the 16s Amplicon sequencing...
Please share ur experiences if anyone came across with such problems
Thank you
Rosh
Comment