SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
Refurbished Hiseq 4000, Nextseq 500, Hiseq 2500v4 & Miseq DNA Sequencers <Sponsored> IET Illumina/Solexa 0 02-01-2018 10:04 AM
Refurbished Hiseq 4000 & Hiseq 2500v4 in-stock IET Illumina/Solexa 0 12-28-2017 12:53 PM
HiSeq 4000 upgrade buthercup_ch Illumina/Solexa 4 07-27-2016 04:49 AM

Reply
 
Thread Tools
Old 05-31-2018, 03:17 PM   #1
kevin199011
Junior Member
 
Location: Austin

Join Date: May 2014
Posts: 4
Default long amplicon on HiSeq 4000

We have generated long amplicon library that is about 1400bp. And their diversity is high. Does anyone have experiences of sequencing such long amplicon on HiSeq4000? Any suggestions are welcomed. Thank you!
kevin199011 is offline   Reply With Quote
Old 05-31-2018, 06:08 PM   #2
luc
Senior Member
 
Location: US

Join Date: Dec 2010
Posts: 346
Default

Is this a one-off experiment or are you planning for multiple samples?

You will need to shear the DNA sample either by sonication or enzymatically to pieces of about 300 to 450 bp length for the Hiseq 4000. Then it can be treated like a genomic DNA sample.
luc is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 07:05 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO