Hello everyone,
I am in the process of deciding what state-of-the-art equipment to buy to setup a new genomics lab, so that I can automate as much of the process of 16s rDNA based bacterial community profiling (for human microbiome studies on samples such as stool and saliva). As I understand, some of the steps involved are:
1. Automating sample addition into DNA extraction kit
2. DNA extraction (right now I am using a Kit, but I'd like to know if there is any machine/equipment that will do this automatically)
3. Amplifying extracted DNA (e.g. PCR machines)
4. Post-PCR purification of amplified DNA and feeding into sequencing machine (e.g. Illumina MiSeq) which will provide all the target 16s rDNA sequences in a centralized server/cloud for subsequent bioinformatics analysis
Any advice on the industry best machines/instruments for each of the above steps (and any additional/intermediate steps I may have missed) would be very helpful. Thanks!
I am in the process of deciding what state-of-the-art equipment to buy to setup a new genomics lab, so that I can automate as much of the process of 16s rDNA based bacterial community profiling (for human microbiome studies on samples such as stool and saliva). As I understand, some of the steps involved are:
1. Automating sample addition into DNA extraction kit
2. DNA extraction (right now I am using a Kit, but I'd like to know if there is any machine/equipment that will do this automatically)
3. Amplifying extracted DNA (e.g. PCR machines)
4. Post-PCR purification of amplified DNA and feeding into sequencing machine (e.g. Illumina MiSeq) which will provide all the target 16s rDNA sequences in a centralized server/cloud for subsequent bioinformatics analysis
Any advice on the industry best machines/instruments for each of the above steps (and any additional/intermediate steps I may have missed) would be very helpful. Thanks!
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