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  • problems when using the software Phrap and consed

    when I use consed to display ace file generated by phrap one error happens:

    The error is :

    exception thrown: ace file error detected from source file parseAceFile.cpp at 2368
    Error in file 34-9EST.out.ace at line 1736. Line:


    premature end of file while expecting DS line for read

    ace file: 34-9EST.out.ace
    Version 20.0 (101012)
    ace file error detected from source file parseAceFile.cpp at 2368
    Error in file 34-9EST.out.ace at line 1736. Line:


    premature end of file while expecting DS line for read
    ace file = 34-9EST.out.ace

    Does anyone know how to solve this problems? thanks !

    my sequence file is a multiple fasta file, I used this command to generate the ace file:

    for the multifasta file, I did not use the phred to generate it. the detail format is like:

    >109823783

    GATCCATCACTTCTACCAGCTTCATCATGCAATAAAACTGTAAGAATCCCTGCACTACCTGCGGCAACAAATGCTCAAAGCAGACTTTACAATGAATATGCTGCAGTATATCTTCTCATCGCTTCTTCATTGACCATGATTATACTCTAGTTCGAGGCTGATTCAGTCTTTTCTCCAGAACTGTATGACATAAGATCAGTCTTCTTAAGGACAAATGGATGAAATCCTCTCAAGTTTAACACTGATTCAGGCATGACCACCTGTTAATGGAATGTTCAACCAGATGCAGACTCAAGTCCATGTATCTGATCATATAACGATTCTTGCATGTTTTCTGTAATAATATTTTTGTAAAAAAAGGCTACTTGGGT

    >84174260

    TCTTCCTCCTCTAGTTTTTCACGTTCCCAAACTCTCCTAACGTCTTAACCATTCTCCTAATCTCTTTTCCATTTTTGGCTTCCTTTAAGCTTTCTTTCCAACCTTACTTGTATTACTAAGTGAAAGAAGAAGATTATGAGTGCAGGCGTGTCTCTCCTCACTATGCTATTCCTTGAGTTGTTTATGCTTTTCGCCTGCTTCCATGAATGTTTATCCAGCCCTGTGGATCAACTTAAAAGTTATATCTTAACACAAAG



    ./phrap 34-9EST.out -new_ace > 34-9test.out

  • #2
    This problem has been "discussed" on the consed mailing list with you ... have you solved the problem or have you switched to MIRA?

    Sven

    Comment


    • #3
      Yes, I am trying to use mira to do my work! thanks for your good advice.

      The reason why I want to post here is that I still want to solve the problem because it is still there.

      But I think MIRA can do a very good similar job.

      Comment

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