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  • OTU picking from illumina asssembly of Human Microbiome data

    Hi All,

    I have 800 gb HQF clean pairend gut illumina data .
    i have assembled the data.
    Now i want to predict Operational taxonomical Units from assembly fasta contigs.
    Apperently none of tool is working well.
    What tool I can go with for OTU picking from assemled data sets.
    Thanks

  • #2
    Maybe you can provide us with a list of tools you tried and why they don't work, since you mentioned you have already tried some!

    Comment


    • #3
      I have 24 assemblies in fasta format generated from velvet 1.2.10
      Tools i have used on assembly
      1. DNAclust
      2. Cd-hit otu Illumina
      3. Ch hit
      Finally I am using mOTU. perl script.
      Which have wide output.

      Comment

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