Hi all, I just completed the Illumina TruSeq RNA v2 protocol and my end product gave a bioanalyzer trace that looks like there's some sort of DNA ladder contamination in the preps. The issue is, no ladder of any sort was used in this protocol so I'm not sure exactly where something like that would come from. Has anyone seen a BA trace like this after a TruSeq prep? I started with 1 ug of whole RNA for each sample and did subsequent purifications with Ampure XP beads, not gel extraction. Any insight would be greatly appreciated.
Thanks
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