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Old 08-16-2012, 08:42 PM   #1
honey
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Default Single cell RNAseq _ Transcriptome sequence

I have data which has ben generated from Single cells (Stem cells/ cancer cells), I was wondering if some one has work flow for such anlaysis or any open source tools if some one has tested the tool.

Thanks
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Old 08-20-2012, 05:49 AM   #2
honey
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Default single cell RNA seq

Any suggestion or experience to share on this?
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Old 08-20-2012, 05:57 AM   #3
Avazeh
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Use Galaxy:
http://galaxy.psu.edu/

There are easy video tutorials for beginners, also nice document and support too!

Have fun
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Old 08-20-2012, 09:48 PM   #4
honey
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Cool Single cell RNA-seq

I use Galaxy a lot, Could you please specify which tool you are referring to for Single cell RNA-seq. Am I missing something?
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Old 08-21-2012, 02:52 AM   #5
Avazeh
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This might be helpful:
https://main.g2.bx.psu.edu/u/fluidig...naseq-workflow

Could you be more specific? What kind of analysis you like to do?
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Old 08-21-2012, 10:43 AM   #6
NextGenSeq
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How did you make the single cell RNA-Seq library? We have problems going below 20 cells.
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