SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
poor postcap yield-Kapa lib prep thiNGS Sample Prep / Library Generation 7 10-24-2014 07:14 PM
Innaccurate Kapa Quant Standards Daytwa Sample Prep / Library Generation 1 02-11-2014 12:09 PM
Kapa Quant Kit-- Roche light cycler sanju0891 454 Pyrosequencing 0 05-07-2012 01:13 AM
FLX vs Titanium, Lib-L vs Lib-A, Amplicon Libraries = confusion SeqTruth 454 Pyrosequencing 4 03-03-2012 12:08 PM
Has anyone used r-quant? quix RNA Sequencing 0 08-24-2010 12:56 PM

Reply
 
Thread Tools
Old 10-08-2019, 08:26 PM   #1
wei210
Junior Member
 
Location: Texas

Join Date: Jul 2019
Posts: 4
Default Kapa lib quant question

Hello. I am new here. I have a naive question. When we make series dilution for kapa lib quant.
1) Will it make big difference if dilute the lib using H2O vs 10mM tris PH8.0 w 0.05% tween-20 ?
2) will kapa conc typically higher than the Qubit conc (lib w Illumina p5/p7 sequence primer) ?
3) vetex to mix should be better than Pipet up/down. In the case using Pipet up/down. How many times up/down should be sufficient?
Thank you all so much
wei210 is offline   Reply With Quote
Old 10-10-2019, 01:11 AM   #2
seq198
Member
 
Location: Germany

Join Date: Dec 2015
Posts: 10
Default

For PCR-free libs kapa quant kit makes sense, as qubit will not deliver accurate results. he different buffers for diluting should not have a big effect for what I can say. 5 times pipetting up and down should be sufficient.
seq198 is offline   Reply With Quote
Old 10-10-2019, 05:18 AM   #3
wei210
Junior Member
 
Location: Texas

Join Date: Jul 2019
Posts: 4
Default

Thank you so much, Seq198
wei210 is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 02:02 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO