Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • How the GQ of a SNP is calculated by UnifiedGenotyper?

    Hi, I'm trying to understand how does UnifiedGenotyper usually calculate the GQ of a SNP.
    I understood the model to calculate the Likelihood of all the genotypes (AA,AC, AG,AT,CC,CG,CT,GG,GT,TT).

    Once all the likelihood have been calculated, correct me if I'm wrong, the likelihood of the best genotype is normilized to 1 and all the other likelihoods are normilezed according to that scale.
    So the PL field in the VCF should be the Phred-scaled values of the normalized values.

    But is not clear to me how do they finally calculate the GQ value.
    What values do they use to calculate that quality (normalized or phred scaled)? And what's the right formula?

    I've tried to debug the code but it ends to be really tricky.
    I really hope that anyone of you know the answer and would help me. I would be really thankfull for that.

  • #2
    The GATK forum would probably be a better place to ask this, the developers of GATK often reply to this sort of question.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Current Approaches to Protein Sequencing
      by seqadmin


      Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
      04-04-2024, 04:25 PM
    • seqadmin
      Strategies for Sequencing Challenging Samples
      by seqadmin


      Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
      03-22-2024, 06:39 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, 04-11-2024, 12:08 PM
    0 responses
    31 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 10:19 PM
    0 responses
    32 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 09:21 AM
    0 responses
    28 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-04-2024, 09:00 AM
    0 responses
    53 views
    0 likes
    Last Post seqadmin  
    Working...
    X