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RNA-Seq: Length Bias Correction for RNA-seq Data in Gene Set Analyses. Newsbot! Literature Watch 0 01-22-2011 03:02 AM

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Old 02-07-2018, 12:16 PM   #1
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Default 5' RNA-Seq Bias?

What are some possible reasons for 5->3' bias in RNA seq data? Libraries were generated using Illumina's TruSeq Stranded mRNA protocol, with pretty good quality RNA input. If anything, with a polyA selection method, I would think there'd be 3' bias.


Last edited by l_zembek; 02-08-2018 at 10:19 AM.
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