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Old 08-27-2013, 11:31 PM   #1
rahbz
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Location: Kharagpur, India

Join Date: Jun 2013
Posts: 16
Default demultiplexing data

Hi,
I am totally new to this field, I have a problem while multiplexing the data. I have downloaded the .fastq files for a particular study from SRA database.
For the same I have manually added the barcode (oligos for mothur) or mapping file (for qiime) and provided the same to MOTHUR and also QIIME.


Paired end fastq files of illumina downloaded from ftp site (http://www.ncbi.nlm.nih.gov/sra/?term=SRR069027) and map file (attached) is prepared from supplementary data of research article. Now while running demultiplexing, none of them are actually being multiplexed.

In QIIME :
split_libraries.py -m map_file_SRR069027/map_file_SRR069027_forward.txt -f SRR069027_1.fna -q SRR069027_1.qual -b 6 -o split_SRR069027_1
In MOTHUR:
make.contigs(ffasta=SRR069026_1_head100000.fasta,rfasta=SRR069026_2_head100000.fasta,rqfile=SRR069026_2_head100000.qual,fqfile=SRR069026_1_head100000.qual,oligos=oligos.mothur.txt)

Thanks in advance,
Attached Files
File Type: txt map_file_SRR069027_corrected.txt (1.7 KB, 28 views)
File Type: pdf Paired-End Illumina Reads Bartam SUPLEMENTARY .pdf (576.5 KB, 27 views)
File Type: txt oligos.mothur.txt (715 Bytes, 15 views)
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