Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Question about --genemodel=complete option of Augustus

    Hi all,

    I am afraid I have a silly question but I am really confused.

    I am now trying to construct a de novo gene model for my species using augustus.

    I ran augustus with "--genemodel=complete" option as below:
    augustus --species=mySpecies --exonnames=on --codingseq=on --protein=on --extrinsicCfgFile=extrinsic.M.RM.E.W.cfg --alternatives-from-evidence=true --hintsfile=hint.gff --allow_hinted_splicesites=atac --genemodel=complete genome.fa > aug.out

    ant then, used getAnnoFasta.pl to extract amino acid sequences in fasta format.
    However, I found in aug.out.aa file that some predicted proteins do not start with methionine(Met).

    Does this kind of thing occur commonly or can it be attribute to my fault at any steps of augustus?

    Best,
    evolver

  • #2
    As far as I know, "complete" means just "not partial". With the default settings, augustus predicts incomplete gene models at the sequence boundaries. This makes sense because the program will be used mainly on draft genomes which consist of a lot of contigs. Besides that, there are also mature proteins that do not start with a Met, so making this a prerequisite might sometimes also be wrong.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Essential Discoveries and Tools in Epitranscriptomics
      by seqadmin


      The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...
      Yesterday, 07:01 AM
    • seqadmin
      Current Approaches to Protein Sequencing
      by seqadmin


      Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
      04-04-2024, 04:25 PM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, 04-11-2024, 12:08 PM
    0 responses
    55 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 10:19 PM
    0 responses
    51 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 09:21 AM
    0 responses
    45 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-04-2024, 09:00 AM
    0 responses
    55 views
    0 likes
    Last Post seqadmin  
    Working...
    X