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Old 12-16-2017, 05:47 PM   #1
XIAOXIAO
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Default Getting rid of primer dimer for RNA-Seq libraries

Hey all, I just found several of my RNA-Seq libraries have primer dimers, I was wondering what is the ratio of my final product that is now diluted in resuspension buffer to AMPure beads to get rid of the primer dimers?
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Old 12-16-2017, 07:32 PM   #2
nucacidhunter
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You will need to do a second clean up with the same bead/DNA ratio used for clean up if you have been following a kit protocol. You will need to do second clean up on all libraries to avoid batch affect.
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Old 12-16-2017, 08:10 PM   #3
XIAOXIAO
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So I used trueseq, they recommend using 1:1 ratio, so can I proceed with 1:1
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Old 12-16-2017, 10:29 PM   #4
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That should be fine, just make sure that your pipette is accurate and use the same pipette for measuring library volume and bead.
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