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Old 01-10-2018, 05:58 PM   #1
kgoglin
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Location: San Diego

Join Date: Dec 2014
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Default Bioanalyzer and qPCR concentrations differ with large libraries

We have a theory that large libraries, specifically those with fragments trailing off to >2kb, do not quantitate accurately from Bioanalzyer. We think this because qPCR concentrations do not correlate. Has anyone else had this problem? It seems if we are able to remove the "tail", then qPCR matches a bit better with Bioanalzyer.
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