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Old 01-11-2018, 04:18 AM   #1
Asashoryu
Junior Member
 
Location: NL

Join Date: May 2009
Posts: 7
Question T over-representation in NextSeq500 run

Hello, we got strange results from some of the recent RNA-seq runs on NextSeq500; example: image001.png

Funny is that we have observed it in some runs while other runs from the same experiment (all processed with NextFlex kit and after polyA selection) but sequenced later worked fine. (RIN was ok, no weird yield after library preparation)

Illumina blaims the sample... What makes sense as the re-run with flowcell from different batch showed same result. But I do not understand what would make such a strange result.

Any thoughts?
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