Dear all,
What's your opinion about this shearing (for ChIP-seq with Illumina)? My settings are: suspension cell-lines, 10 min PFA crosslinking 1%, 5 min of quenching with glycine, 30 cycles of shearing (Bioruptor, 30 s on/ 30 s off). Is this shearing good enough for sequencing? I have calculated that approx 54 % of my DNA is at the correct size while approx 43 % is too large. Is it possible to get one peak of 100-500 bp with crosslinked material?
What's your opinion about this shearing (for ChIP-seq with Illumina)? My settings are: suspension cell-lines, 10 min PFA crosslinking 1%, 5 min of quenching with glycine, 30 cycles of shearing (Bioruptor, 30 s on/ 30 s off). Is this shearing good enough for sequencing? I have calculated that approx 54 % of my DNA is at the correct size while approx 43 % is too large. Is it possible to get one peak of 100-500 bp with crosslinked material?
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