Cost per run on GAII

Guy · 06-04-2009, 12:31 PM

Does anyone know the current cost per run for the GAII, in terms of consumables etc.? How much does it change if you're just doing one lane? Any help would be awesome.

james hadfield · 06-04-2009, 11:58 PM

Hi,
Here are my numbers which I give absolutely no guarantees on! They are a pretty good indication of the consumables needed per lane including flow cell and sequencing reagents. All costs assume one lane is used for PhiX and the other lanes share that cost. The numbers in brackets are what we currently exepct to get from a 36bp kit. We will wait and see what formats the new kits will come in, here's hoping for a 150bp sequencing kit!
I hope you are all as stunned as I was when I saw that a 2x150bp lane costs only £1450 (£10K per flow cell). This brings the possibility of nearly 20M 250bp contigs using a 2x150bp read from a 250bp insert library. What can you NOT do with that?

1 lane of SE sequencing without library prep 36bp (44) £ 375
1 lane of SE sequencing without library prep 72bp (88) £ 500
1 lane of SE sequencing without library prep 108bp (132) £ 650
1 lane of SE sequencing without library prep 144bp (176) £ 750

1 lane of PE sequencing without library prep 36bp (44) £ 700
1 lane of PE sequencing without library prep 72bp (88) £ 950
1 lane of PE sequencing without library prep 108bp (132) £ 1200
1 lane of PE sequencing without library prep 144bp (176) £ 1450

Guy · 06-05-2009, 02:20 AM

Thanks for the info, James. Greatly appreciated.

Do people know if James' info is in line with what users end up paying in the US? Do they quote out lower prices for volume users?

Chipper · 06-05-2009, 03:18 AM

What is the current cost of fragment library preps and number of sequences per lane on good runs?

james hadfield · 06-05-2009, 07:01 AM

up to 18M reads on GA2, should be almost 20M on a good day from GA2x pipeline 1.4.
Library preps are about £250 per sample from Illumina but the oligo only kit can reduce this substantially. It is very dependant on which kit you want to use.

Chien-Yuan Chen · 06-05-2009, 03:01 PM

We cost about USD 1400 dollars per lane with 75bpXII PE sequencing per lane.

captainentropy · 06-22-2009, 04:58 PM

I think the core facility I've been using charges us about $450 per lane. I was told it will increase though, perhaps about 10%.

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06-04-2009, 12:31 PM	#1
Guy Junior Member Location: America Join Date: Jun 2009 Posts: 2	Cost per run on GAII Does anyone know the current cost per run for the GAII, in terms of consumables etc.? How much does it change if you're just doing one lane? Any help would be awesome.

06-04-2009, 11:58 PM	#2
james hadfield Moderator Cambridge, UK Community Forum Location: Cambridge, UK Join Date: Feb 2008 Posts: 221	Hi, Here are my numbers which I give absolutely no guarantees on! They are a pretty good indication of the consumables needed per lane including flow cell and sequencing reagents. All costs assume one lane is used for PhiX and the other lanes share that cost. The numbers in brackets are what we currently exepct to get from a 36bp kit. We will wait and see what formats the new kits will come in, here's hoping for a 150bp sequencing kit! I hope you are all as stunned as I was when I saw that a 2x150bp lane costs only £1450 (£10K per flow cell). This brings the possibility of nearly 20M 250bp contigs using a 2x150bp read from a 250bp insert library. What can you NOT do with that? 1 lane of SE sequencing without library prep 36bp (44) £ 375 1 lane of SE sequencing without library prep 72bp (88) £ 500 1 lane of SE sequencing without library prep 108bp (132) £ 650 1 lane of SE sequencing without library prep 144bp (176) £ 750 1 lane of PE sequencing without library prep 36bp (44) £ 700 1 lane of PE sequencing without library prep 72bp (88) £ 950 1 lane of PE sequencing without library prep 108bp (132) £ 1200 1 lane of PE sequencing without library prep 144bp (176) £ 1450

06-05-2009, 02:20 AM	#3
Guy Junior Member Location: America Join Date: Jun 2009 Posts: 2	Thanks for the info, James. Greatly appreciated. Do people know if James' info is in line with what users end up paying in the US? Do they quote out lower prices for volume users?

06-05-2009, 03:18 AM	#4
Chipper Senior Member Location: Sweden Join Date: Mar 2008 Posts: 324	What is the current cost of fragment library preps and number of sequences per lane on good runs?

06-05-2009, 07:01 AM	#5
james hadfield Moderator Cambridge, UK Community Forum Location: Cambridge, UK Join Date: Feb 2008 Posts: 221	up to 18M reads on GA2, should be almost 20M on a good day from GA2x pipeline 1.4. Library preps are about £250 per sample from Illumina but the oligo only kit can reduce this substantially. It is very dependant on which kit you want to use.

06-05-2009, 03:01 PM	#6
Chien-Yuan Chen Member Location: USA Join Date: Feb 2009 Posts: 19	We cost about USD 1400 dollars per lane with 75bpXII PE sequencing per lane.

06-22-2009, 04:58 PM	#7
captainentropy Member Location: San Francisco Bay Area Join Date: Mar 2009 Posts: 89	I think the core facility I've been using charges us about $450 per lane. I was told it will increase though, perhaps about 10%.