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BEADS vs PYICOS
Hi,
I have ChIP-seq SOLiD sequencing data with a relatively low amount of reads and I would like to extend the length of the fragments.
My reads are 75 nucleotides long and the DNA fragments were around 300 bp after sonication. Would you recommend to use BEADS or PYICOS ?
BEADS has some other bias removal options (GC content, mappability, structure). How much does this improve the quality of the data ?
I have ChIP-seq SOLiD sequencing data with a relatively low amount of reads and I would like to extend the length of the fragments.
My reads are 75 nucleotides long and the DNA fragments were around 300 bp after sonication. Would you recommend to use BEADS or PYICOS ?
BEADS has some other bias removal options (GC content, mappability, structure). How much does this improve the quality of the data ?