Hello,
I have, this morning, set up a Next-Seq run for some ChIP-seq libraries. It is now on its 39th cycle. However, the cluster PF value is 75.94 yet the %≥Q30 is at 90.82. I have around 150,000,000 reads per lane...
I was wondering is this a case of over-clustering? If so, are the reads not as usable?
I have, this morning, set up a Next-Seq run for some ChIP-seq libraries. It is now on its 39th cycle. However, the cluster PF value is 75.94 yet the %≥Q30 is at 90.82. I have around 150,000,000 reads per lane...
I was wondering is this a case of over-clustering? If so, are the reads not as usable?
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