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Old 01-14-2013, 08:46 PM   #1
Jed C.
Junior Member
 
Location: Corvallis, Oregon

Join Date: Jan 2013
Posts: 1
Exclamation biom from QIIME to MEGAN

Greetings all,

I was wondering if someone could help me with an issue importing a .biom file from QIIME into MEGAN. All works well when I import the OTU table (.biom) initially, however it doesn't work after my manual manipulations of the OTU table... here is my process...

1. Create OTU table from pick_otus.py and assign_taxonomy.py
2. Convert the OTU table (.biom) to a tab delimited text file which I manually edited in Excel (some taxonomies were suspect and lacking so I manually edited them based on other BLAST results.
3. I set up the OTU table to look EXACTLY like the old tab delimited one and convert it back to a .biom file but MEGAN will not import any taxonomic stream or OTU quantities.

Any help for formatting this correctly to use in MEGAN would be greatly appreciated. I can provide further details if necessary. Thanks.
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