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Old 09-30-2009, 12:06 AM   #1
edge
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Default Difference between .sff files and .fq file

Anybody knows what is the main difference between the .sff file and .fq file?
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Old 09-30-2009, 12:44 AM   #2
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.fq file: http://maq.sourceforge.net/fastq.shtml
.sff file: http://blog.malde.org/index.php/2008...binary-format/
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Old 09-30-2009, 01:30 AM   #3
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thanks a lot

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Old 10-04-2009, 06:30 PM   #4
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Quote:
Originally Posted by edge View Post
Anybody knows what is the main difference between the .sff file and .fq file?
A .SFF file contains the FLOWGRAMS from the 454 pyrosequencing platform. The are sort of like a "run length encoding" of the genomic base pairs, and very application/platform specific.

A .FQ is a FASTQ file which contains genomic base pair information and associated quality scores. It is a generic file format.

You can process .sff down to .fq, but going the other way is not really meaningful.
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