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Old 12-09-2010, 08:22 AM   #1
Location: Russia, Moscow

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Default Mate-Paired kit for Fragment library sequencing

Hello, all! Please help me, can I use TOP Mate-Paired Seq kit (MM50) for Fragment library sequencing (MM50). Should I use F3-tag strip (with added Focal Map reagent from R3-tag strips)?
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Old 12-10-2010, 10:18 AM   #2
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I think that should work fine. Pretty sure the focal map reagent anneals to the P1 and P2 parts of the amplicons. F3 mate-pair and F3 fragment should be the same primers (anchored in the P1 adaptor.)

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Old 12-16-2010, 05:42 PM   #3
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F3(fragment) and F3(mate-pair) are the same, i don't think there is any problem
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