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Old 02-15-2012, 05:50 PM   #1
moedog45
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Location: Washingto, D.C.

Join Date: Feb 2012
Posts: 4
Default Presence of RNA for Sureselect XT

Hello,

I have some gDNA samples that have a sufficient amount of DNA according to the Qubit fluorometer and also have a 260/280 ratio of about 1.85-1.90. However, I'm concerned that there may still be some RNAs in these samples because they were not treated with RNAse during sample prep. Would the presence of RNA seriously impact any aspect of the SureSelect XT protocol? If so, are there any suggestions about how best to treat these samples with RNAse?
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Old 02-16-2012, 10:08 AM   #2
liliniu
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Location: Boston, MA

Join Date: Aug 2010
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Default

Hi moedog45:

You may contact SureSelect support for your question.

Here are the contact information:
SureSelect Support
Phone 800-227-9770 x 3 x 4 x 4 (North America) Email sureselect.support@agilent.com

Quote:
Originally Posted by moedog45 View Post
Hello,

I have some gDNA samples that have a sufficient amount of DNA according to the Qubit fluorometer and also have a 260/280 ratio of about 1.85-1.90. However, I'm concerned that there may still be some RNAs in these samples because they were not treated with RNAse during sample prep. Would the presence of RNA seriously impact any aspect of the SureSelect XT protocol? If so, are there any suggestions about how best to treat these samples with RNAse?
liliniu is offline   Reply With Quote
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