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  • Isoform quantification, sanity check

    Hi!

    Im studying adipose and skeletal muscle tissue atm. As a quick sanity check of Cuffdiff results on isoforms, I want to look at well described isoforms.

    What I mean is to look at i.e. two isoforms the occur in a fixed ratio within the tissue. And then to see if the RNAseq results also quantifies the same ratio.

    Does anyone know of such examples for skeletal muscle/adipose tissue, or have other sanity check methods (both dry- and wet-lab based).

    Thank you!

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