Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Improving PCR-free NGS library preparation

    PCR-free NGS libraries reduce bias, improve coverage of difficult areas, and enhance the detection of sequence variants. However, the current PCR-free NGS library prep kits tend to have low sequencing yields than that of PCR amplified libraries because:

    • Full libraries are mixed with partially ligated libraries and un-ligated DNA fragments, thus making it difficult to optimize library quantity due to inaccuracies in library size and amount.
    • Partially ligated libraries and un-ligated DNA fragments reduce cluster efficiency.

    In order to address this issue, BioDynami has developed the PCR-free NGS DNA Library Prep Kit.



    Our PCR-free kit effectively removes partially ligated libraries and un-ligated DNA fragments, resulting in only fully ligated DNA fragments in the final library. The maximal sequencing yield can be reached since cluster density is optimized based on accurate library sizes and amounts. Also, there is no clustering interference caused by partially ligated libraries and un-ligated DNA fragments.



    Features of BioDynami PCR-free NGS kit:

    • Pure, full library: Contains only fully ligated DNA fragments
    • Fast: Hands-on time around 10 min; Total time less than 2 hrs
    • Simple workflow: Two reaction steps for library construction; Simple reaction setup with master mix solution for reaction steps

    More details at: https://biodynami.com/product/pcr-fr...brary-prep-kit

    ---
    Last edited by BioDynami; 09-08-2020, 07:51 AM.

Latest Articles

Collapse

  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM
  • seqadmin
    Techniques and Challenges in Conservation Genomics
    by seqadmin



    The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

    Avian Conservation
    Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
    03-08-2024, 10:41 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Yesterday, 06:37 PM
0 responses
10 views
0 likes
Last Post seqadmin  
Started by seqadmin, Yesterday, 06:07 PM
0 responses
9 views
0 likes
Last Post seqadmin  
Started by seqadmin, 03-22-2024, 10:03 AM
0 responses
51 views
0 likes
Last Post seqadmin  
Started by seqadmin, 03-21-2024, 07:32 AM
0 responses
67 views
0 likes
Last Post seqadmin  
Working...
X