Hello,
I tried to assemble a mitochondrial genome assembly with SPAdes assembler using pair-end Illumina and Nanopore sequencing data. The size of the Illumina data is 18 GB and when I do hybrid assembly after trimming adapters, it showing that Memory is insufficient to run this program and requires at least 389 GB RAM. But, I am having 188 GB RAM.
Then, I used BBmerge commands to sort out this issue. But when I run pair-end reads using bbmerge-auto.sh command, its saying that "try increasing the -Xmx flag and using tool specific memory related parameters".
Please suggest me how to do hybrid assembly with both Illumina and Nanopore data?
I tried to assemble a mitochondrial genome assembly with SPAdes assembler using pair-end Illumina and Nanopore sequencing data. The size of the Illumina data is 18 GB and when I do hybrid assembly after trimming adapters, it showing that Memory is insufficient to run this program and requires at least 389 GB RAM. But, I am having 188 GB RAM.
Then, I used BBmerge commands to sort out this issue. But when I run pair-end reads using bbmerge-auto.sh command, its saying that "try increasing the -Xmx flag and using tool specific memory related parameters".
Please suggest me how to do hybrid assembly with both Illumina and Nanopore data?
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