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Can I multiplex bacterial genomes on MiSeq? If so, how many ? Thank you!
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Uh...how much coverage will be useful for you?
Today = 8-9M PF read pairs (2x150bp from each) = ~2.6GB = ~566x coverage of one E.coli sized genome (4.5MB). Divide at will. -
What does PF stand for? Sorry, I'm a newbie in NGS.Comment
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It means Pass Filter - not all reads generated pass the filtering process eg a run could generate 10 million reads but only 9 million are passed by the quality filtering process.Originally posted by eosin View PostComment
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...05-06-2024, 07:48 AM -
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