Hello,
the group I am working in is preparing for some targeted (~50 genes) analysis of variations on tumor samples using the Ion Torrent platform. As the number of chips needs to be minimized due to cost, we were trying to design the experiment to obtain the best results out of our limitations.
This brings us to the problem: determining the optimal coverage depth per mutation frequency. In other words, what is a good coverage depth to detect mutations with these frequencies:
Is there any publication or reference where I can get an ideal grasp of this so we can adjust the experiment design accordingly? Thanks in advance.
the group I am working in is preparing for some targeted (~50 genes) analysis of variations on tumor samples using the Ion Torrent platform. As the number of chips needs to be minimized due to cost, we were trying to design the experiment to obtain the best results out of our limitations.
This brings us to the problem: determining the optimal coverage depth per mutation frequency. In other words, what is a good coverage depth to detect mutations with these frequencies:
- 20%
- 10%
- 5%
Is there any publication or reference where I can get an ideal grasp of this so we can adjust the experiment design accordingly? Thanks in advance.
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