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Old 07-16-2018, 05:27 PM   #1
genetic03
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Location: South Korea

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Default Barcoding for cfDNA?

Hello,

We will use barcoding system for cfDNA WES (both cancer and normal).
In addition to cfDNA WES, we will perform tissue WES (same patients).
My question is, if we do barcoding system for cfDNA WES, should we use barcoding system for tissue WES?

Thank you in advance.
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Old 07-17-2018, 12:44 AM   #2
nucacidhunter
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Barcoding in this context is vague. I wonder if you mean using indexed adapters for multiplex sequencing.
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Old 07-17-2018, 12:50 AM   #3
genetic03
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Quote:
Originally Posted by nucacidhunter View Post
Barcoding in this context is vague. I wonder if you mean using indexed adapters for multiplex sequencing.
Yes. Barcoding here means indexing.
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Old 07-17-2018, 01:08 AM   #4
nucacidhunter
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Using indexes would give more options for multiplex sequencing with no adverse effect.
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