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Old 07-25-2018, 08:07 AM   #1
ragan1
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Location: NW Indiana

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Default I am trying to do a qPCR analysis of a miRNA in the rat brain that needs to control f

I am trying to do a qPCR analysis of a miRNA in the rat brain that needs to control for treatment, sex, and siblings and I'm having trouble w/stats. Usually I do the delta delta ct method, but having the within-litter comparison, plus treatment, plus controlling for the endogenous control is making this challenging.
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