Hi all,
I'm a new member in this forum. I'm having trouble with preparation of my sample using Nextera XT kit. Most of my samples came out with too low concentration after PCR cleaningup. I checked my starting DNA conc (e.g for 2.25ng/ul, I used 0.44ul sample). I don't have problem with my cleanup process but the library yield is always coming out too little for sequencing. Please, I'd appreciate anyone with valuable suggestions.
Thanks
I'm a new member in this forum. I'm having trouble with preparation of my sample using Nextera XT kit. Most of my samples came out with too low concentration after PCR cleaningup. I checked my starting DNA conc (e.g for 2.25ng/ul, I used 0.44ul sample). I don't have problem with my cleanup process but the library yield is always coming out too little for sequencing. Please, I'd appreciate anyone with valuable suggestions.
Thanks
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