Hi,
I was wondering if anyone could help with the optimal cycling conditions for the post-adapter-ligated amplification step (using PE adapters) in the Sure Select Target Enrichment system? Given the short and long PCR primers required, is a sort of 2-step PCR required?
Additionally if one were to use the "homemade" adapters, are the adapters required to be pre-annealed before the amplification? How are these "Y" -shaped adapters generated?
Any help would be great,
Thanks,
Luke.
I was wondering if anyone could help with the optimal cycling conditions for the post-adapter-ligated amplification step (using PE adapters) in the Sure Select Target Enrichment system? Given the short and long PCR primers required, is a sort of 2-step PCR required?
Additionally if one were to use the "homemade" adapters, are the adapters required to be pre-annealed before the amplification? How are these "Y" -shaped adapters generated?
Any help would be great,
Thanks,
Luke.
Comment