Hi,
I am a bit puzzled and after searching here and with google, I strangely couldn't find an answer. Maybe because it's Friday and I can't see the forest for the trees.
What is the actual size of the small RNA adapters?
In the manual of the TruSeq small RNA library prep kit it says that "The band containing the 22 nt RNA fragment with both adapters are a total of 147 nt." This would mean that the adapters add 125 nt. That's also what you typically see with Bioanalyzer, Fragment Analyzer, etc.
However, when I merge the sequences from the customer sequence letter, I only get 118 nt. What am I missing here?
5' AATGATACGGCGACCACCGAGATCTACAC GTTCAGAGTTCTACAGTCCGACGATC (insert) TGGAATTCTCGGGTGCCAAGG AACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTG 3'
5'-adapter and 3'-adapter
index
Thanks.
I am a bit puzzled and after searching here and with google, I strangely couldn't find an answer. Maybe because it's Friday and I can't see the forest for the trees.
What is the actual size of the small RNA adapters?
In the manual of the TruSeq small RNA library prep kit it says that "The band containing the 22 nt RNA fragment with both adapters are a total of 147 nt." This would mean that the adapters add 125 nt. That's also what you typically see with Bioanalyzer, Fragment Analyzer, etc.
However, when I merge the sequences from the customer sequence letter, I only get 118 nt. What am I missing here?
5' AATGATACGGCGACCACCGAGATCTACAC GTTCAGAGTTCTACAGTCCGACGATC (insert) TGGAATTCTCGGGTGCCAAGG AACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTG 3'
5'-adapter and 3'-adapter
index
Thanks.