Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • TruSeq small RNA adapter size

    Hi,

    I am a bit puzzled and after searching here and with google, I strangely couldn't find an answer. Maybe because it's Friday and I can't see the forest for the trees.

    What is the actual size of the small RNA adapters?
    In the manual of the TruSeq small RNA library prep kit it says that "The band containing the 22 nt RNA fragment with both adapters are a total of 147 nt." This would mean that the adapters add 125 nt. That's also what you typically see with Bioanalyzer, Fragment Analyzer, etc.
    However, when I merge the sequences from the customer sequence letter, I only get 118 nt. What am I missing here?

    5' AATGATACGGCGACCACCGAGATCTACAC GTTCAGAGTTCTACAGTCCGACGATC (insert) TGGAATTCTCGGGTGCCAAGG AACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTG 3'

    5'-adapter and 3'-adapter
    index

    Thanks.

Latest Articles

Collapse

  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin




    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
    04-22-2024, 07:01 AM
  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Yesterday, 08:47 AM
0 responses
12 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
60 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
59 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 09:21 AM
0 responses
54 views
0 likes
Last Post seqadmin  
Working...
X