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Old 08-30-2017, 03:20 PM   #1
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Default Return not aligned with BLAST


I am new to Seqanswers, though not to Bioinformatics.

I have a doubt with BLAST. Is there a simple way to get all the sequences, after blasting one database to another database, that have not been aligned (under a e-value threshold)?
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Old 08-31-2017, 02:48 PM   #2
Brian Bushnell
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If you can grab the headers of the sequences that were aligned, you can use BBMap's or a similar tool to remove them from the source fasta.
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blast, blast+

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