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Old 09-01-2017, 05:49 AM   #1
Location: canada

Join Date: Nov 2010
Posts: 20
Default Is it OK to use paired-end 150bp to sequence ATAC-seq libraries?


I am planning to do ATAC-seq assays and I am wondering if it is fine to use paired-end 150bp to sequence ATAC-seq libraries?
Just because the sequencing platform I am working with is only doing 150bp run with HiSeq3000...
I have the impression that people are usually using PE sequencing with 50 to 75bp reads. Would the only problem be that I would sequence a lot of adapters (exept money lost, adapter trimming should be enough to solve this)? Or would I lost many "small" fragments?

Thank you for your help!
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