Go Back   SEQanswers > Applications Forums > RNA Sequencing

Similar Threads
Thread Thread Starter Forum Replies Last Post
RNA-Seq: Differential expression--the next generation and beyond. Newsbot! Literature Watch 0 01-03-2012 02:40 AM
RNA-Seq: Differential expression in RNA-seq: A matter of depth. Newsbot! Literature Watch 0 09-10-2011 02:12 AM
RNA-Seq: From RNA-seq reads to differential expression results. Newsbot! Literature Watch 0 12-24-2010 02:13 AM
RNA-Seq: Cloud-scale RNA-sequencing differential expression analysis with Myrna. Newsbot! Literature Watch 0 08-13-2010 08:00 AM

Thread Tools
Old 01-17-2011, 06:46 AM   #1
Location: Mexico city

Join Date: Jul 2008
Posts: 12
Default RNA-seq for differential expression

We would like to start some RNA-seq experiments for quantitation and differential expression but I am not sure how deep should we sequence a sample to have enough coverage and have a good estimation of the transcripts
meaning: Should we sequence 1 or 2 lanes per sample or can we multiplex several samples in one lane?
This point is crucial since we have several samples and cost is very important!
The organism is Mouse and we have a GA IIx.
Has anybody done this kind of experiments that can share some advice?
Inti is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 11:01 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO